J Integr Plant Biol. ›› 1997, Vol. 39 ›› Issue (4): 296-301.

• Research Articles • Previous Articles     Next Articles

Access and Identification of Intergeneric Hybrid Between Brassica juncea×Crambe abyssinica

Wang You-ping, Luo Peng, Li Xu-feng and Lan Ze-qu   

  • Published:1997-04-20

Abstract: An intergeneric hybrid between Brassica juncea (L.) Coss. x Crambe abyssinica Hochst. ex R. E. Fries was obtained for the first time through routine method combined with embryo rescue. The inductivity of hybrids was 2.1%. In many respects the Fl hybrid resembled either its maternal or paternal parent br both. Consequenfiy, retarded growth, wrinkled leaves and rudimentary anthers in Fl plant were observed. Fl pollen varied in size and 10% ~ 15% were of the paternal pollen type,i.e. ,4-colpate. Fl pollen viability was low and 12% ~ 25% of pollens was viable, with a mean of 18.5%. The chromosome number of Fl somatic cell was 63 which was intermediate between the two parents. Most of pollen mother cells had 63 chromosomes. Analysis of isozymes further confirmed that the F1 hybrid combined the paternal characteristic bands.

Key words: Crambe abyssinica, Brassica juncea, Intergeneric hybrid, Identification

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