Construction and Analysis of cDNA Libraries from Proembryos and just Differentiating Young Embryos in Rice

Abstract

Abstract:

Two cDNA libraries were constructed from microdissected 214 rice proembryos (2-3 d after pollination) and 121 just differentiating young embryos (3-5 d after pollination) respectively through RT-PCR technique. The primary libraries had a total of 3.7×10 phages for the proembryos and a total of 2.5×10 phages for the just differentiating young embryos, in which 96% of the phages were recombinants. Insert sizes ranging from 400 bp to 3?500 bp were obtained. All of theabove mentioned accorded with the general requirements of cDNA library construction.

水稻原胚和刚启动分化的幼胚cDNA文库的构建与分析
陈绍荣李师弢吕应堂杨弘远*

（武汉大学生命科学学院发育生物学研究中心，武汉430072）
关键词：水稻；原胚；分化胚；cDNA文库

Key words: rice, proembryo, differentiating embryo, cDNA library

CHEN Shao-Rong, LI Shi-Tao, Lü Ying-Tang, YANG Hong-Yuan. Construction and Analysis of cDNA Libraries from Proembryos and just Differentiating Young Embryos in Rice[J]. J Integr Plant Biol., 2000, 42(2): 214-216.

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Construction and Analysis of cDNA Libraries from Proembryos and just Differentiating Young Embryos in Rice

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