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J Integr Plant Biol, 2000, 42 (9): 988-990, Research Article
Detection of Differentiation Among BB, CC and EE Genomes in the Genus Oryza by Two-probe Genomic in situ Hybridization (GISH)
Chang-Bao , ZHANG Da-Ming, GE Song, LU Bao-Rong, HONG De-Yuan
doi:
Abstract

双探针原位杂交揭示稻属BB、cc 和EE 基因组之间的分化
李常宝1 张大明1* 葛颂1  卢宝荣2 洪德元1

(1. 中国科学院植物研究所系统与进化植物学开放研究实验室,北京100093;2.Genetiic Resources Center, International Rice Research Institute, MCPO Box 3127, 1271 Makati City,Philippines)

摘要:利用双探针原位杂交技术 ,揭示了稻属Oryzaofficinalis复合体中BB、CC和EE基因组之间的分化。以标记的BB基因组 (来自二倍体的O .punctataKotechyexSteud .)的总DNA为探针 ,同BBCC基因组 (O .minutaJ.S .Presl.etC .B .Presel)的中期染色体杂交。结果表明 ,BB基因组的DNA探针同与四倍体O .minuta中的BB基因组的染色体之间有强烈的杂交信号 ,而与CC基因组的染色体之间的杂交信号很弱。而EE基因组 (来自O .aus traliensisDomin)的总DNA标记的探针同O .minuta基因组的所有染色体均有不同程度的杂交 ,且杂交信号的大小和强弱在BB基因组的染色体和CC基因组的染色体之间无显著的差异。由此证明 :(1)CC和BB基因组之间的分化程度很大 ;(2 )EE基因组同CC和BB基因组具有一定的亲缘关系 ;(3)CC基因组与EE基因组之间的关系较近而同BB基因组关系较远。

关键词: 基因组原位杂交(CISH);稻属;基因组分化;BBCC 基因组;BB基因组;EE基因组

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