J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (3): 854-875.DOI: 10.1111/jipb.13399

• Plant Biotic Interactions • Previous Articles    

The NAC transcription factor ONAC083 negatively regulates rice immunity against Magnaporthe oryzae by directly activating transcription of the RING-H2 gene OsRFPH2-6

Yan Bi1, Hui Wang1, Xi Yuan1,2, Yuqing Yan1, Dayong Li1 and Fengming Song1*   

  1. 1. National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China;
    2. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China
    *Correspondence: Fengming Song (fmsong@zju.edu.cn)
  • Received:2022-07-01 Accepted:2022-10-26 Online:2022-10-29 Published:2023-03-01

Abstract: NAC transcription factors (TFs) play critical roles in plant immunity by modulating the expression of downstream genes via binding to specific cis-elements in promoters. Here, we report the function and regulatory network of a pathogen- and defense phytohormone-inducible NAC TF gene, ONAC083, in rice (Oryza sativa) immunity. ONAC083 localizes to the nucleus and exhibits transcriptional activation activity that depends on its C-terminal region. Knockout of ONAC083 enhances rice immunity against Magnaporthe oryzae, strengthening pathogen-induced defense responses, and boosting chitin-induced pattern-triggered immunity (PTI), whereas ONAC083 overexpression has opposite effects. We identified ONAC083-binding sites in the promoters of 82 genes, and showed that ONAC083 specifically binds to a conserved element with the core sequence ACGCAA. ONAC083 activated the transcription of the genes OsRFPH2-6, OsTrx1, and OsPUP4 by directly binding to the ACGCAA element. OsRFPH2-6, encoding a RING-H2 protein with an N-terminal transmembrane region and a C-terminal typical RING domain, negatively regulated rice immunity against M. oryzae and chitin-triggered PTI. These data demonstrate that ONAC083 negatively contributes to rice immunity against M. oryzae by directly activating the transcription of OsRFPH2-6 through the ACGCAA element in its promoter. Overall, our study provides new insight into the molecular regulatory network of NAC TFs in rice immunity.

Key words: ACGCAA element, immunity, Magnaporthe oryzae, ONAC083, OsRFPH2-6, rice

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