J Integr Plant Biol. ›› 2001, Vol. 43 ›› Issue (11): 1207-1210.

• Research Articles • Previous Articles    

Preparation and Application of Monoclonal Antibodies Specific for Salicylic Acid (in English)

WANG Shu-Cai, LI Guo-Jing, XIA Kai, XU Lang-Lai, CHEN Pu-Yan and ZHOU Xie   

Abstract:

专一识别水杨酸的单克隆抗体的制备及应用
王树才1,2  李国婧1 夏凯1 徐朗莱 陈溥言3 周燮1*

(1.  南京农业大学农学院,南京210095;2. 南京农业大学理学院,南京210095;#$ 南京农业大学动物医学院,南京210095)

摘要:首次报道了专一识别水杨酸(salicylic acid, SA)的单克隆抗体的制备.该抗体源于以5- 氨基水杨酸(5-ASA)的5位氨基为偶联位点,血蓝蛋白(KLH)为载体合成的SA-NH-CH2-NH- KL H 免疫原.它对 SA 和 5-ASA 具有高亲和力.用该抗体建立了定量测定 SA 的直接酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA),其检测范围为 0.02~20 nmol.利用该 SA ELISA,研究了黄瓜(Cucumis sativus L.)叶片接种丁香假单胞菌(Pseudomonas syringae pv. syringae van Hall)后 1~ 32 h之间游离态SA含量的动态变化,发现其变化趋势呈双峰曲线.

关键词: 水杨酸;单克隆抗体;酶联免疫吸附测定法;黄瓜

Key words: salicylic acid, monoclonal antibody, enzyme-linked immunosorbent assay, Cucumis sativus

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