Abstract:

Using BrUTP incorporation and statistical analysis, the authors investigated RNA transcription in the interphase nuclei in Physarum polycephalum Schw. The rRNA synthesis in the nucleolus continued actively throughout the interphase, while in nucleoplasm hnRNA was transcribed at low levels in S phase and actively in G2 phase. The rate of nucleoplasm RNA transcription was doubled between early S phase and late S phase, and a 5-6 folds increase was detected between the early S phase and G2 phase.

多头绒泡菌间期细胞核中RNA 的转录状况
焦明大*毕晓辉 曾庆华 王玉红
（东北师范大学遗传与细胞研究所，长春130024）

摘要：利用BrUTP免疫标记技术 ,研究了多头绒泡菌 (Physarumpolycephalum Schw .)间期细胞核中RNA的转录状况。结果表明 :在整个间期核仁中的rRNA都在活跃转录 ;核质中hnRNA的转录呈逐渐上升趋势 ,早S期转录水平很低 ,晚S期转录活性升高 1倍 ,G₂ 期转录达到最高水平 ;整个间期核质中RNA的转录水平增加了 5～ 6倍
关键词：rRNA；hnRNA转录；BrUTP；S 期；G₂期；多头绒泡菌

Key words: rRNA, hnRNA, transcription, BrUTP, S phase, G2 phase, Physarum polycephalum