J Integr Plant Biol. ›› 2001, Vol. 43 ›› Issue (4): 435-437.

• Research Articles • Previous Articles    

Establishment of in Vitro Regeneration System of Triploid Chinese White Poplar

LU Shan-Fa, ZHAO Hua-Yan, WEI Jian-Hua, SONG Yan-Ru   

Abstract:

三倍体毛白杨组织培养再生系统的建立
卢善 发赵华燕*  魏建华 宋艳茹**

(中国科学院植物研究所,北京100093)

摘要:初步建立了三倍体毛白杨(Populus tomentosa Carr.)组织培养再生系统。组织培养时,外植体最好选用在弱光下从休眠芽上萌发的幼芽或嫩茎,经HgCl2溶液表面消毒后,接种到大量元素减半、含0.25mg/L吲哚乙酸和1.00mg/L玉米素的MS培养基上。试管苗的生根可采用含0.25mg/L吲哚丁酸和10mg/L维生素B1的MS培养基。试管苗茎段的分化依无性系和外源激素条件的不同而异。无性系B19的茎段较易分化,而无性系B304和B331分化较难。最适的分化培养基为大量元素减半、含0.05~0.10mg/L吲哚乙酸和1.00mg/L玉米素的MS培养基。

关键词: 三倍体;毛白杨;组织培养

Key words: triploid, Populus tomentosa, tissue culture

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