Abstract:

A linkage map consisting of 221 markers was constructed based on a recombinant inbred line (RIL) population from the cross between Zhenshan 97 and Minghui 63. Quantitative trait loci (QTL) mapping was carried out for grain appearance traits such as grain length, grain width and grain shape in rice in 1998 and 1999. Based on interval mapping method at the threshold LOD≥2.4, six, two and two QTLs were detected for grain length, grain width and grain shape, respectively, in 1998; In 1999, three, two and two QTLs were identified for the three traits, respectively. Of them, seven QTLs were simultaneously identified in both of the years. The QTL with large effects located in the interval RG393-C1087 on chromosome 3 not only controlled the grain length, but also influenced the grain shape. It explained 57.5%, 61.4% and 26.7%, 29.9% of phenotypic variation of the grain length and the grain shape in two years, respectively. The QTL with large effects located in the interval RG360-C734B on chromosome 5 affected the grain width and the grain shape. It explained 44.2%, 53.2% and 32.1%, 36.0% of phenotypic variation of the grain width and the shape in two years, respectively. Eight, five and five QTLs were identified for the grain length, width and shape, respectively, based on mixed linear-model composite interval mapping method at P =0.005. Their general contributions were 58.81%, 44.75%, and 57.47%. One QTL for the grain length was found to be significant interaction with environment.

利用水稻重组自交系群体定位谷粒外观性状的数量性状基因
邢永忠 谈移芳 徐才国 华金平 孙新立
（华中农业大学作物遗传改良国家重点实验室，武汉430070）

摘要：用区间作图和混合线性模型的复合区间作图两种方法 ,对水稻 (Oryzasativa L .)珍汕 97和明恢 6 3组合的重组自交系群体的谷粒外观性状———粒长、粒宽和粒形进行了数量性状基因 (QTL)定位。用区间作图法在LOD≥ 2 .4水平上 (近似于α =0 .0 0 5) ,1 998年对粒长、粒宽和粒形分别检测到 6、2和 2个QTLs ;1 999年对以上 3个性状分别检测到 3、2和 2个QTLs。其中 7个QTLs在两年均检测到。位于第 3染色体RG393-C1 0 87区间的QTL效应大 ,同时影响粒长和粒形 ,两年贡献率分别为 57.5%、6 1 .4%和 2 6 .7%、2 9.9%。位于第 5染色体RG36 0 -C734B区间的QTL效应大 ,同时影响粒宽和粒形 ,两年贡献率分别为 44.2 %、53.2 %和 32 .1 %、36 .0 %。用混合线性模型的复合区间作图法在P =0 .0 0 5水平上 ,对粒长、粒宽和粒形分别检测到 8、5和 5个QTLs ,共解释各自性状变异的 58.81 %、44.75%和 57.47%。只检测到 1个QTL与环境之间存在显著互作。

关键词： 水稻；重组自交系群体；粒长；粒宽；粒形；数量性状基因座位

Key words: rice, recombinant inbred line population, grain length, grain width, grain shape, quantitative trait loci