J Integr Plant Biol. ›› 2004, Vol. 46 ›› Issue (4): 423-427.

• Research Articles • Previous Articles     Next Articles

A Circular Dichroism Spectroscopic Study Revealing the Cause of the Changes of Chlorophyll a Fluorescence Induction of Photosystem Ⅱ During Heat Treatment

LI Dong-Hai, RUAN Xiang, XU Qiang, GONG Yan-Dao, ZHANG Xiu-Fang, ZHAO Nan-Ming WANG Ke-Bin, KUANG Ting-Yun   

Abstract:

Chlorophyll a fluorescence and circular dichroism (CD) spectra of photosystem Ⅱ (PSⅡ) membrane were measured after heat treatment. The chlorophyll fluorescence parameter Fo'' remained stable after treatment at the temperatures from 30 ℃ to 40 ℃ and then reached a maximum after treatment at 55 ℃. In PSⅡ membranes and LHCⅡ (light-harvesting chlorophyll a/b binding complex)-enriched complexes, anomalous CD signals with extremely large amplitudes occurred during the heat treatment. The temperature corresponding to the maximum anomalous CD intensity peaking at 677 nm was 40 ℃. The results indicate that the aggregation state of the LHCⅡ in PSⅡ is related to the anomalous CD signal, and can be an important factor influencing Fo'' in the heat treatment of PSⅡ membrane.

Key words: chlorophyll, circular dichroism (CD), photosystem Ⅱ (PSⅡ), light-harvesting chlorophyll a/b binding complex (LHCⅡ)

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