Abstract:

A bacterial artificial chromosome library has been constructed for Triticum boeoticum Boiss（AbAb）using the bacterial artificial chromosome (BAC)vector pECBAC1. The library consists of about 170 000 clones. A random sampling analysis of 200 BAC clones indicates that the average insert size is 104 kb. Based on the genome size of T. boeoticum, the library is about three timesas large as T. boeoticum haploid genome (5 600 Mb). Screening the BAC library with cpDNA sequence psbA gene and mtDNA sequence atp6 gene as probe shows that contamination of the library with chloroplast and mitochondrial clones is less than 1%. The library will be a useful platform in gene clone and genomic research of wheat.

野生一粒小麦BAC文库的构建和鉴定

陈凡国^1,2  张学勇^1*  夏光敏²  贾继增¹

(1.中国农业科学院品种资源研究所,农业部作物种质资源与生物技术重,点实验室,北京,100081；2.山东大学生命学院,济南,250100)

以细菌人工染色体pECBAC1为载体,构建了野生一粒小麦(Triticum boeoticum B oiss)的基因组BAC文库.该文库共包含约17万个克隆,平均插入片段长度为104 kb,按野生一粒小麦基因组为5 600 Mb计算,文库覆盖了约3倍的该物种基因组.用大麦叶绿体psb A基因和玉米线粒体atp6基因作混合探针,检测发现该文库中含细胞器基因组同源序列的克隆数小于1% .该文库的建成,为小麦基因的克隆及基因组学研究提供了技术平台.

关键词 野生一粒小麦 BAC文库 基因组

*通讯作者 Email: xueyongz@public.bta.net.cn

Key words: Triticum boeoticum, BAC library, genome