J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (5): -.

• Research Articles •    

Purification and Amino-terminal Sequencing of a 68 kD Glycopolypeptide of the Plasma Membrane from Maize Sperm Cells

XU Heng-Ping, JIN Cheng, YANG Shou-Jun, CAO Zong-Xun (TSAO T H) and ZHANG Shu-Zheng   

Abstract: Based on author's previous work on detection and immunolocalization of glycoproteins of the plasma membrane of maize ( Zea mays L. ) sperm cells, a 68 kD peripheral specific glycopolypeptide of the plasma membrane from maize sperm cells was purified by IEF-SDS two-dimensional electrophoresis. It presents specif- ically positive reaction in Con A-HRP (concanavalin A-horseradish peroxidase) staining, and its pi value is 5.5. The search in protein sequence database reveals that the amino-terminal sequence of this glycopolypeptide is identical with that of Con A. But its difference from Con A in molecular weight and pi value indicates that it could be related to a Con A receptor on the plasma membranes of maize sperm cells instead of being Con A itself. It is fascinating to study further the function of the above glycopolypeptide in gametic recognition, adhesion and fusion of the double fertilization in maize.

Key words: Specific glycopeptide, Amino-terminal sequence, Zea mays, Sperm cell, Plasma membrane

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