J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (3): -.

• Research Articles •    

Transformation and Expression of Trichosanthin Gene in Tomato

JIANG Guo-Yong, JIN De-Min, WENG Man-Li, GUO Bao-Tai and WANG Bin   

Abstract: Trichosanthin (TCS) is a type-I ribosome inactivating protein. Previous studies indicate that TCS has antivirus effect on HIV, HBV, TMV and many other viruses. The authors report that TCS gene was integrated into binary transformation vector pBI121-2 and a new recombinant plasmid pBI121TG24 containing the recombinant TCS-GUS gene was obtained, pBI121TG24 was transferred into Agrobacterium tumefaciens strain LBA4404 and a new genetic engineered strain pBI121TG24/LBA4404 was obtained, pBI121TG24/LBA4404 was used to transform the tomato ( Lycopersicon esculentum Mill. ) eultivar "Peak" and "Meitin 11" by the conmmon leaf-disc method. Results indicated that TCS gene was integrated into tomato genomic DNA evidenced with assays of kanamycin screening X-glu detection, PCR amplification, dot blot and Southem blot. The transformation efficiency of TCS gene was 1.13% for "Peak" and 0.72% for "Meiyin 11". The transgenic plant TP3, TP11 have shown virus-resistance on TMV and CMV.

Key words: Trichosanthin gene, Transformation, Transgenie tomato

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