J Integr Plant Biol. ›› 1990, Vol. 32 ›› Issue (3): -.

• Research Articles •    

Simultaneous Determination of Indole-3-Acetic Acid and Abscisic Acid as the Pentafluorobenzyl Derivative with Electron-Capture Gas Chromatography

Li Hui-lin, Yun Zi-hou,Yang Yi-ping, Zhang Li-ping and Tan Zhi-yi   

Abstract: Indole-3-acetic acid (IAA) and abscisic acid (ABA) were converted in to pentafluorobenzyl esters by α-bromo-2, 3, 4, 5, 6-pentafluorotoluene at 55℃. The derivatization took about 90 minutes. The two esters generated were able to be simultaneously determined with electron-capture gas chromatography. The method is simple and sensitive. The minimal test does: 10-14g for IAA and 10-13g for ABA.

Key words: Derivatization, Indole-3-acetic acid, Abscisic acid, α-bromo-2 ,3 ,4 ,5 ,6-Pentafluorotoluene

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