J Integr Plant Biol. ›› 1989, Vol. 31 ›› Issue (12): -.

• Research Articles •    

Plantlet Regeneration from Protoplasts lsolated from an Embryogenic Suspension Cultureof Cotton(Gossypium hirsutum L.)

Chen Zhi-xian, Li Shu-jun, Yue Jian-xiong, Jiao Gai-li, Liu Shao-xiang, She Jian-ming, Wu Jing-yin and Wang Hai-bo   

Abstract: Protoplasts were isolated from an embryogenic suspension culture of commercial cotton cv. The protoplasts were released enzymatically and isolated by centrifugation on a sucrose cushion. The isolated protoplasts were initially cultured in a liquid medium with K3 mineral salts and modified Km8p organic compositions, supplemented with 0.05–0.1 mg/l 2,4-D, 0.2–0.5 mg/l 2ip in the dark. The regenerated plantlets from protoplasts of coker312 and coker 201 cv. were obtained. Embryogenesis from protoplast of Jin4 cv. and microcolonies form protoplasts of JiHe321 and Lul cv. were observed.

Key words: Culture of protoplasts, plantlet regenerauon, Cotton

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