Abstract: Leaf explants of Rubus laciniatus were cultured on Nitsch and Nitsch basic medium supplemented with either 6-BA 2 mg/L and NAA 0.1 kg/l or 2, 4-D 2.5 mg/l and NAA 0.1 mg/l. Adventitious buds were produced from leaf-like structures. Some ultrastructural observations of cells have shown that the most easy change part of a plastid during the cell differentiation in Rubus leaf explant culture in vitro was lamella system, storage starch and plastogluboli.

Key words: Rubus laciniatus, Organogenesis, Plastid, Photolamella, Starch grain, Leaf-like structure