J Integr Plant Biol. ›› 2011, Vol. 53 ›› Issue (8): 598-607.DOI: 10.1111/j.1744-7909.2011.01041.x

Special Issue: Rice Genomics and Agriculture

• Cell and Developmental Biology •     Next Articles

Identification of a Stable Quantitative Trait Locus for Percentage Grains with White Chalkiness in Rice (Oryza sativa)

Tao Guo1, Xiaolu Liu2, Xiangyuan Wan1, Jianfeng Weng1, Shijia Liu1, Xi Liu1, Mingjiang Chen1, Jingjing Li1, Ning Su2, Fuqing Wu2, Zhijun Cheng2, Xiuping Guo2, Cailin Lei2, Jiulin Wang2, Ling Jiang1* and Jianmin Wan1,2*   

  1. 1Key Laboratory of Crop Genetics and Germplasm Enhancement/ Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China
    2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Abstract:

High chalkiness is a major problem in many rice-producing areas of the world, especially in hybrid rice (Oryza sativa L.) in China. We previously showed a major quantitative trait locus for the percentage of grains with white chalkiness (QTLqPGWC-8) in the interval G1149-R727 on chromosome 8 using a chromosome segment substitution line (CSSL). Here, we selected the line-CSSL50 harboring the QTLqPGWC-8 allele from the CSSLs derived from a cross between Asominori (as a recurrent parent) and IR24 (as a donor parent), which had higher percentage chalkiness, markedly different from that of Asominori. There were also significant differences in starch granules, appearance of amylose content (AAC) and milling qualities between Asominori and CSSL50, but not in grain size or thousand grain weight (TGW). The BC4F2 and BC4F3 populations from a cross between CSSL50 and Asominori were used for fine mapping of qPGWC-8. We narrowed down the location of this QTL to a 142 kb region between Indel markers 8G-7 and 8G-9. QTLqPGWC-8 accounted for 50.9% of the difference in PGWC between the parents. The markers tightly linked to qPGWC-8 should facilitate cloning of the gene underlying this QTL and will be of value for marker-assisted selection in breeding rice varieties with better grain quality.

Guo T, Liu X, Wan X, Weng J, Liu S, Liu X, Chen M, Li J, Su N, Wu F, Cheng Z, Guo X, Lei C, Wang J, Jiang L, Wan J (2011) Identification of a stable quantitative trait locus for percentage grains with white chalkiness in rice (Oryza sativa) J. Integr. Plant Biol. 53(8), 598–607.

Key words: fine mapping, marker-assisted selection, percentage grains with white chalkiness, QTL, rice

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