J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (10): 965-978.DOI: 10.1111/jipb.12058

• Molecular Physiology • Previous Articles     Next Articles

Comparative Proteomic Analysis of Rice Shoots Exposed to High Arsenate

Yanli Liu1,2, Ming Li2, Chao Han2, Fengxia Wu1, Bingkun Tu1 and Pingfang Yang2*   

  1. 1College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
    2Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Moshan, Wuhan 430074, China
  • Received:2013-02-26 Accepted:2013-04-09 Published:2013-06-17
  • About author:*Corresponding author Tel.: +86 27 8751 0956; Fax: +86 27 8751 0956; E-mail: yangpf@wbgcas.cn

Abstract:

Consumption of arsenic contaminated water and cereals is a serious threat to humans all over the world. Rice (Oryza sativa “Nipponbare”), as a main cereal crop, can accumulate arsenic more than 10-fold that of in other cereals. To gain a comprehensive understanding of the response of rice subjected to 100 µM arsenate stress, a comparative proteomic analysis of rice shoots in combination with morphological and biochemical investigations have been performed in this study. The results demonstrated that arsenate suppressed the growth of rice seedlings, destroyed the cellular ultra-structure and changed the homeostasis of reactive oxygen species. Moreover, a total of 38 differentially displayed proteins, which were mainly involved in metabolism, redox and protein-metabolism, were identified. The data suggest the arsenic can inhibit rice growth through negatively affecting chloroplast structure and photosynthesis. In addition, upregulation of the proteins involved in redox and protein metabolism might help the rice to be resistant or tolerant to arsenic toxicity. In general, this study improves our understanding about the rice arsenic responsive mechanism.

Liu Y, Li M, Han C, Wu F, Tu B, Yang P (2013) Comparative proteomic analysis of rice shoots exposed to high arsenate. J. Integr. Plant Biol. 55(10), 965–978.

Key words: Rice, arsenate, proteomics, ultra-structure, metabolism

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22