J Integr Plant Biol. ›› 2014, Vol. 56 ›› Issue (4): 388-399.DOI: 10.1111/jipb.12149

Special Issue: Cell Biology and Functions

• Functional Omics and Systems Biology • Previous Articles     Next Articles

Identification, occurrence, and validation of DRE and ABRE Cis‐regulatory motifs in the promoter regions of genes of Arabidopsis thaliana

Sonal Mishra1†, Aparna Shukla2†, Swati Upadhyay1, Sanchita1, Pooja Sharma2, Seema Singh1, Ujjal J. Phukan1, Abha Meena2, Feroz Khan2, Vineeta Tripathi3, Rakesh Kumar Shukla1* and Ashok Shrama1   

  1. 1Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, A Laboratory Under Council of Scientific and Industrial Research, Lucknow, India
    2Metabolic and Structural Biology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, A Laboratory Under Council of Scientific and Industrial Research, Lucknow, India
    3Botany Department, CSIR-Central Drug Research Institute (CSIR-CDRI), Lucknow, India
  • Received:2013-11-27 Accepted:2013-12-12 Published:2013-12-20
  • About author:Authors Contributed equally.
    *Corresponding Author: E-mail: rk.shukla@cimap.res.in

Abstract:

Plants posses a complex co-regulatory network which helps them to elicit a response under diverse adverse conditions. We used an in silico approach to identify the genes with both DRE and ABRE motifs in their promoter regions in Arabidopsis thaliana. Our results showed that Arabidopsis contains a set of 2,052 genes with ABRE and DRE motifs in their promoter regions. Approximately 72% or more of the total predicted 2,052 genes had a gap distance of less than 400 bp between DRE and ABRE motifs. For positional orientation of the DRE and ABRE motifs, we found that the DR form (one in direct and the other one in reverse orientation) was more prevalent than other forms. These predicted 2,052 genes include 155 transcription factors. Using microarray data from The Arabidopsis Information Resource (TAIR) database, we present 44 transcription factors out of 155 which are upregulated by more than twofold in response to osmotic stress and ABA treatment. Fifty-one transcripts from the one predicted above were validated using semiquantitative expression analysis to support the microarray data in TAIR. Taken together, we report a set of genes containing both DRE and ABRE motifs in their promoter regions in A. thaliana, which can be useful to understand the role of ABA under osmotic stress condition.

Mishra S, Shukla A, Upadhyay S, Sanchita, Sharma P, Singh S, Phukan UJ, Meena A, Khan F, Tripathi V, Shukla RK, Shrama A (2014) Identification, occurrence and validation of DRE and ABRE cis‐regulatory motifs in the promoter regions of genes of Arabidopsis thaliana. J Integr Plant Biol 56: 388–399. doi: 10.1111/jipb.12149

Key words: Abscisic acid, abscisic acid-responsive elements, dehydration-responsive element, osmotic stress response

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22