J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (5): 486-495.DOI: 10.1111/jipb.12249

• Molecular Physiology • Previous Articles     Next Articles

Calcium contributes to photoprotection and repair of photosystem II in peanut leaves during heat and high irradiance

Sha Yang1,2†, Fang Wang1,3†, Feng Guo1,2, Jing-Jing Meng1,2, Xin-Guo Li1,2* and Shu-Bo Wan2*   

  1. 1Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, China
    2Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, China
    3College of Life Sciences, State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
  • Received:2014-06-26 Accepted:2014-08-07 Published:2014-08-07
  • About author:These authors contributed equally to this work.
    **Correspondence: E-mail: lixinguo@tom.com; wansb@saas.ac.cn

Abstract:

In this study, we investigated the effects of exogenous calcium nitrate on photoinhibition and thylakoid protein level in peanut plants under heat (40 °C) and high irradiance (HI) (1,200 µmol/m2 per s) stress. Compared with control seedlings (cultivated in 0 mmol/L Ca(NO3)2 medium), the maximal photochemical efficiency of photosystem II (PSII) in Ca2+-treated plants showed a slight decrease after 5 h stress, accompanied by lower degree of PSII closure (1-qP), higher non-photochemical quenching, and lower level of membrane damage. Ca2+ inhibitors were used to analyze the varieties of antioxidant enzymes activity and PSII proteins. These results indicated that Ca2+ could protect the subunits of PSII reaction centers from photoinhibition by reducing the generation of reactive oxygen species. In the presence of both ethyleneglycol-bis(2-aminoethylether)-tetraacetic acid and ascorbic acid (AsA), the net degradation of the damaged D1 protein was faster than that only treated with AsA. Our previous study showed that either the transcriptional or the translational level of calmodulin was obviously higher in Ca2+-treated plants. These results suggested that, under heat and HI stress, the Ca2+ signal transduction pathway can alleviate the photoinhibition through regulating the protein repair process besides an enhanced capacity for scavenging reactive oxygen species.

 

Yang S, Wang F, Guo F, Meng JJ, Li XG, Wan SB (2015) Calcium contributes to photoprotection and repair of photosystem II in peanut leaves during heat and high irradiance. J Integr Plant Biol 57: 486–495 doi: 10.1111/jipb.12249

Key words: Calcium, D1 protein, heat and high irradiance stress, peanut, photosystem II

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