J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (7): 719-723.

• Research Articles • Previous Articles     Next Articles

)Genetic Transformation of Tomato with Snowdrop Lectin Gene (GNA)

WU Chang-Yin, YE Zhi-Biao, LI Han-Xia, TANG Ke-Xuan   

Abstract:

The plasmid pRSSGNA1 carried a snowdrop lectin gene (Galanthus nivalis agglutinin, GNA) under the drive of RSs-1 promoter, were successfully transferred into three tomato (Lycopersicon esculentum Mill.) cultivars, “C8”,“A39” and “A53” via Agrobacterium-mediated transformation. Regenerated plantlets were obtained from cotyledons after preculture, shoot inducing culture and root inducing culture. Transgenic tomato plants were confirmed by the kanamycin-resistant experiment, PCR analysis and Southern blot. The preliminary results from bioassay demonstrated significant resistance of the transgenic plants to aphid (Myzus persicae Sulzer) larvae. The inheritance of selective marker gene (NPTⅡ) in 3 transgenic tomato plants is in the model of the simple Mendel's fashion in progenies of the selfing generation.

雪花莲外源凝集素基因转化番茄
吴昌银1 叶志彪1*  李汉霞1 唐克轩2

(1. 华中农业大学作物遗传改良国家重点实验室,武汉430070;2.  复旦大学遗传研究所,上海200433)


摘要:将含有雪花莲外源凝集素基因 (GNA)的质粒pRSSGNA1通过冻融法转化到根癌土壤杆菌 (Agrobacteriumtumefaciens (SmithetTownsend)Conn)菌株LBA44 0 4中。采用叶盘法转化番茄 (Lycopersiconesculentum Mill.)栽培品种“C8”、“A3 9”和“A53 ” ,获得了含GNA基因的 43株转化植株。通过卡那霉素抗性鉴定、NPTⅡ基因PCR和GNA基因Southernblot分析表明 ,GNA基因已整合到番茄的基因组中。初步抗蚜虫 (MyzuspersicaeSulzer)试验证明 ,转基因番茄有一定的抗蚜虫效果。 3株转基因植株的NPTⅡ基因在自交子代中的分离比例符合 3∶1孟德尔遗传规律。

关键词: 番茄;雪花莲外源凝集素基因;遗传转化;蚜虫

Key words: tomato, snowdrop lectin gene (GNA), genetic transformation, aphid

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