Abstract:

The line of T240 6 was selected from 32 SC2 lines of immature embryo culture of T240 （common wheat ( Triticum aestivum L.)× Wheat Haynaldia villosa (L.) Schur. 6D/6V substitution line）through powdery mildew characterization, glutamate oxaloacetate transaminase (GOT) enzyme and gliadin (Gli) analyses and in situ hybridization. All of the individual plants resistant to powdery mildew lacked the locus of GOT at 6V L arm (GOT V2) and had gliadin locus at 6VS arm (Gli-V2) of Haynaldia villosa. All the plants resistant to powdery mildew had one or two telocentric chromosomes that did not pair with wheat chromosomes but paired between themselves. T240-6 was identified as a telocentric line through in situ hybridization. 

幼胚培养创造抗白粉病簇毛麦6VS端体

李辉^1,2  陈孝¹  辛志勇¹  徐惠君¹  杜丽璞¹  马有志¹

(1.中国农业科学院作物育种栽培研究所农业部作物遗传育种重点实验室,北京 100081; 2.河北省农林科学院粮油作物研究所,石家庄 050031)

通过白粉病抗性鉴定、生化标记及分子原位杂交相结合的方法,从小麦(Triticum aestivum L.)幼胚培养组合T240 (普通小麦×小麦-簇毛麦(Haynaldia villosa Lam.) 6D/6V异代换系)的32个SC2代株系中,筛选出T240-6株系,其所有的抗白粉病单株均缺失簇毛麦6V染色体长臂上的谷草转氨酶位点GOT-V2,而具有短臂上的醇溶蛋白位点Gli-V2.细胞学染色体观察表明,该株系的所有抗病单株均具有1～2个端体,这些端体不能与小麦染色体配对,双端体之间可以配对.经原位杂交分析,端体杂交呈阳性,表明它们均为簇毛麦6V染色体短臂(6VS).

关键词 簇毛麦 幼胚培养 端体 谷草转氨酶 醇溶蛋白 原位杂交

Key words: Haynaldia villosa, immature embryo culture, telocentric chromosome, glutamate oxaloacetate transaminase, gliadin, in situ hybridization