J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (2): 120-123.

• Research Articles •     Next Articles

Photochemical Efficiency of PSⅡand Membrane Lipid Peroxidation in Leaves of indica and japonica Rice (Oryza sativa) Under Chilling Temperature and Strong Light Stress Conditions

JI Ben-Hua, ZHU Su-Qin and JIAO De-Mao   


Relationships between fluorescence parameters and membrane lipid peroxidation in leaves of indica and japonica rice (Oryza sativa L.) during later growth stage were studied under chilling temperature and strong light stress conditions. Results showed that D1 protein contents of PSⅡ in photosynthetic app aratus dropped, the generation of antheraxanthin (A) and zeaxanthin (Z)of xanthophyll cycle were inhibited partly, PSⅡ photochemical efficiency (Fv/Fm)and non photochemical quenching (qN) were also decreased obviously. In addition, endogenous active oxygen scavenger—superoxide dismutase (SOD) reduced, superoxide anion radical (O[SX(B-*3)-[]·[SX]]2) and malondialdehyde (MDA) accumulated, as a result, photooxidation of leaves occurred under chilling temperature and strong light stress conditions. Obvious differences in the changes of the above mentioned physiological parameters between indica and japonica rice were observed. Experiments in leaves treated with inhibitors under chilling temperature and strong light conditions showed that indica rice was more sensitive to chilling temperature with strong light and subjected to photooxidation more than japonica rice. Notable positive correlation between D1 protein contents and Fv/Fm or (A+Z)/(A+Z+V), and a marked negative correlation between Fv/Fm and MDA contents were obtained by regression analysis in indica and japonica rice during chilling temperature and strong light conditions. According to the facts mentioned above, it was inferred that PSⅡ photochemical efficiency(Fv/Fm) was the key index to forecast for the prediction of photooxidation under stress circumstances and the physiological basis were the synthetic capacity of D1 protein and the protection of xanthophyll cycle.


季本华1,2  朱素琴2  焦德茂1*

(1. 江苏农业科学院农业生物遗传生理研究所,南京 210014; 2. 南通师范学院生命科学与技术系,南通 226007)

摘要:研究了温光胁迫下籼(Oryza sativa L. spp. indica)粳稻(O. sativa L. spp. japonica)生育后期叶片荧光参数和膜脂过氧化的关系.结果表明,低温强光下水稻光合机构中PSⅡ的D1蛋白量下降,叶黄素循环组分中环氧玉米黄质(A)和玉米黄质(Z)的形成受抑,PSⅡ光化学效率(Fv/Fm)和非光化学猝灭(qN)明显下降.加之内源活性氧清除剂超氧物歧化酶(SOD)活性降低,超氧阴离子自由基(O(-)/(*)2)和膜脂过氧化产物丙二醛(MDA)积累增加,导致光氧化发生.上述过程在籼粳稻间有明显差异,低温强光结合抑制剂处理证明,籼稻较粳稻对低温强光敏感和光氧化严重.相关分析表明,D1蛋白量分别与Fv/Fm(A+Z)/(A+Z+V)呈极显著的正相关关系,Fv/FmMDA呈极显著的负相关关系.据此认为,逆境下Fv/Fm 是预测光氧化的

关键词 D1蛋白 PSⅡ光化学效率(Fv/Fm) 膜脂过氧化 叶黄素循环 水稻

*通讯作者 Email: photosyn@public1.ptt.js.cn


Key words: D1 protein, PSⅡ photochemical efficiency (Fv/Fm), membrane lipid peroxidation, xanthophyll cycle, rice

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q