J Integr Plant Biol. ›› 1996, Vol. 38 ›› Issue (11): -.

• Research Articles •    

Isolation and Photosynthesis of Spheroplasts in Spirulina platensis

Peng Guo-hong, Shi Ding-ji, Fei Xiu-geng and Zeng Cheng-kui (Tseng Cheng-kui)   

Abstract: Spirulina platensis is a nonheterocystic filamentous blue-green alga (cyanobacterium). Large quantity of highly qualified spheroplasts were obtained by improved isolation method. The spheroplast has a wrinkled and porous surface. Their diameter ranged from 3.8 btm to 4. 6 μm. The activity of photosynthetic oxygen evolution in the spheroplasts was about 40 % of the intact cell. The absorption spectra of the filaments and spheroplasts at room temperature revealed that they had the same pigments, Chla, PC, PEC and carotenoid. In spheroplasts the relative content of PC and carotenoid decreased, and that of PEC increased. It implicated that the light absorption of Spirulina platensis could be influenced by the cell wall. Some differences existed between the original cells and spheroplasts in the low temperature fluorescence emission spectra. F757 of spheroplasts excited by 436 nm was reduced obviously and that excited by 580 nm was disapeared. F728/F685 and F640/F685 enhanced, and F693/F685 was reduced. F728/F640 was lower than that of the original cells. These results indicated that removing the cell wall may inhibit the PS Ⅱ activity and influence the F695 from core antenna pigment system.

Key words: Spirulina platensis, Spheroplasts: Photosynthetic oxygen evolution, Fluorescence

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