J Integr Plant Biol. ›› 1996, Vol. 38 ›› Issue (3): -.

• Research Articles •    

Promotion of Carrot Suspension Cell Transformation and Plant Regeneration byAgrobacterium Harboring Binary Vector Pretreated with Phenolic Compounds

Liu Ming-zhi   

Abstract: Non-tumorigenic Agrobacterium tumefaciens strain LBA4404 harboring binary vector pretreated with acetosyringone and complex phenolic compounds significantly increased the rate of carrot (Daucus carota) suspension cell transformation and plant regeneration. Kanamycin resistant colones were selected on media containing 50 mg/L kanamycin. The author's experimental data indicated that transformation frequencies in the group activated by acetosyringone and complex phenolic compounds pretreatment were 0.24% and 0. 17% respectively while that in the phenolic compound non-pretreated was only 0.02%. Transgenic carrot plants were obtained from the resistant colones via somatic embryogenesis on hormone-free media containing 50 mg/L kanamycin. Histochemical analysis showed that high levels of GUS activity appeared in the parenchymatous cells of vascular bundles of roots, stems, leaves and petioles as well as stomatic guard cells, cortical cells, mesophyllous cells and trichome of leaves, also epidermal cells also parenchymatous cells under the epiderm of petiols.

Key words: Daucus carota, Suspension culture cells, Agrobacterium tumefaciens, Genetic transformation, Phenolic compounds, Binary vector, GUS

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