Involvement of Calcium-dependent Protein Kinases in ABA regulation of Stomatal Movement

Abstract

Abstract: Patch-clamp techniques were employed to investigate if calcium-dependent protein kinases (CDPKs) be involved in the signal transduction pathways of stomatal movement regulation by the phytohormone abscisic acid (ABA) in Vicia faba. Stomatal opening was completely inhibited by external application of 1 μmol/L ABA, and such ABA inhibition was significantly reversed by the addition of CDPK inhibitor trifluoper- azine (TFP). The inward whole-cell K+-currents were inhibited by 60% in the presence of 1 μmoL/L intracellular ABA, and this inhibition was completely abolished by the addition of CDPK competitive substrate histone Ⅲ-S. The results suggest that CDPKs may be involved in the signal transduction cascades of ABA-regulated stomatal movements.

Key words: Calcium-dependent protein kinases (CDPKs), K+-channels, Abscisic acid, Stomata] guard cells

WANG Xi-Qing and WU Wei-Hua. Involvement of Calcium-dependent Protein Kinases in ABA regulation of Stomatal Movement[J]. J Integr Plant Biol., 1999, 41(5): -.

[1]	Kenan Jia, Baozhen Zeng, Zhengrong Dai, Xiaoming Zhang, Jing Wang, Chuanbao Wu, Chen Feng, Guohua Yan, Kaichun Zhang, Xuwei Duan, Wei Wang. A signaling pathway mediating abscisic acid-induced jasmonic acid biosynthesis during strawberry ripening [J]. J Integr Plant Biol., 2026, 68(3): 777-792.
[2]	Yike Zeng, Wei Xiao, Yue Wang, Xiangming Shang, Peng Xiao, Jing Qu, Yilei Wang, Xi Zeng, Haowei Chen, Xin Jiang, Chunlong Li, Ji-Hong Liu. ABA signaling orchestrates SnRK1α-dependent phosphorylation of WRKY41 to regulate SPS4 and sugar accumulation in citrus fruit under drought conditions [J]. J Integr Plant Biol., 2026, 68(1): 57-74.
[3]	Hainan Tian, Lu Xu, Xin Li, Yuelin Zhang. Salicylic acid: The roles in plant immunity and crosstalk with other hormones [J]. J Integr Plant Biol., 2025, 67(3): 773-785.
[4]	Chen Zhu, Tian Sang, Zhen Zhang, Yubei Wang, Zhen Lin, Wei Wang, Zhaobo Lang, Jian-Kang Zhu, Pengcheng Wang. Maintaining basal B-RAF kinase activity for abscisic acid signaling via reciprocal phosphoregulation of a single serine residue [J]. J Integr Plant Biol., 2025, 67(11): 2848-2862.
[5]	Patricia Benito, Marina Celdrán, Javier Bellón, Vicente Arbona, Miguel González-Guzmán, Rosa Porcel, Lynne Yenush, José M. Mulet. The combination of a microbial and a non-microbial biostimulant increases yield in lettuce (Lactuca sativa) under salt stress conditions by up-regulating cytokinin biosynthesis [J]. J Integr Plant Biol., 2024, 66(10): 2140-2157.
[6]	Hongliang Wang, Yubei Wang, Tian Sang, Zhen Lin, Rongxia Li, Weiwei Ren, Xin Shen, Bing Zhao, Xiao Wang, Xuebin Zhang, Shaoqun Zhou, Shaojun Dai, Honghong Hu, Chun‐Peng Song and Pengcheng Wang. Cell type-specific proteomics uncovers a RAF15-SnRK2.6/OST1 kinase cascade in guard cells [J]. J Integr Plant Biol., 2023, 65(9): 2122-2137.
[7]	Fengping Du, Yinxiao Wang, Juan Wang, Yingbo Li, Yue Zhang, Xiuqin Zhao, Jianlong Xu, Zhikang Li, Tianyong Zhao, Wensheng Wang and Binying Fu. The basic helix-loop-helix transcription factor gene, OsbHLH38, plays a key role in controlling rice salt tolerance [J]. J Integr Plant Biol., 2023, 65(8): 1859-1873.
[8]	Xuening Wei, Yuyan Li, Xiuliang Zhu, Xin Liu, Xingguo Ye, Miaoping Zhou and Zengyan Zhang. The GATA transcription factor TaGATA1 recruits demethylase TaELF6‐A1 and enhances seed dormancy in wheat by directly regulating TaABI5 [J]. J Integr Plant Biol., 2023, 65(5): 1262-1276.
[9]	Xiaoyun Zhao, Tianren Zhang, Li Bai, Shuangshuang Zhao, Yan Guo, Zhen Li. CKL2 mediates the crosstalk between abscisic acid and brassinosteroid signaling to promote swift growth recovery after stress in Arabidopsis [J]. J Integr Plant Biol., 2023, 65(1): 64-81.
[10]	Sujittra Promchuea, Yujuan Zhu, Zhizhong Chen, Jing Zhang, and Zhizhong Gong. ARF2 coordinates with PLETHORAs and PINs to orchestrate ABA-mediated root meristem activity in Arabidopsis [J]. J Integr Plant Biol., 2017, 59(1): 30-43.
[11]	Bingxian Chen, Jun Ma, Zhenjiang Xu, and Xiaofeng Wang. Abscisic acid and ethephon regulation of cellulase in the endosperm cap and radicle during lettuce seed germination [J]. J Integr Plant Biol., 2016, 58(10): 859-869.
[12]	Liang Yang, Qiaohong Liu, Zhibin Liu, Hao Yang, Jianmei Wang, Xufeng Li, and Yi Yang. Arabidopsis C3HC4-RING finger E3 ubiquitin ligase AtAIRP4 positively regulates stress-responsive abscisic acid signaling [J]. J Integr Plant Biol., 2016, 58(1): 67-80.
[13]	Haitao Shi, Tiantian Ye, Fan Yang, and Zhulong Chan. Arabidopsis PED2 positively modulates plant drought stress resistance [J]. J Integr Plant Biol., 2015, 57(9): 796-806.
[14]	Patrícia Vidigal, Ana Montserrat Martin-Hernandez, Cèlia Guiu-Aragonés, Sara Amâncio, and Luísa Carvalho. Selective silencing of 2Cys and type-IIB Peroxiredoxins discloses their roles in cell redox state and stress signaling [J]. J Integr Plant Biol., 2015, 57(6): 591-601.
[15]	Ji-Hee Min, Jung-Sung Chung, Kyeong-Hwan Lee, and Cheol Soo Kim. The CONSTANS-like 4 transcription factor, AtCOL4, positively regulates abiotic stress tolerance through an abscisic acid-dependent manner in Arabidopsis [J]. J Integr Plant Biol., 2015, 57(3): 313-324.

Involvement of Calcium-dependent Protein Kinases in ABA regulation of Stomatal Movement

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments