Abstract:

The techniques of in situ hybridization (ISH) are widely adopted for analyzing the genetic make-up and RNA expression patterns of individual cells. There are four main criterions for evaluating this technique, including detection sensitivity, resolution, capacity and specificity. This review focuses on a number of advances made over the last years in the fluorescence in situ hybridization (FISH). These advances can be catagorized into several branches as follows: (1) Multicolor-FISH (mFISH), including conventional mFISH, combinatorial FISH, ratio labelling FISH, multicolor chromosome painting and comparative genomic hybridization (CGH); (2) Extended DNA fiber-FISH (EDF-FISH), including quantitative DNA fiber mapping (QDFM), molecular combing (MC) and dynamic molecular combing (DMC); (3)In situ PCR-based FISH; (4) Bacterial (or yeast) artificial chromosome-FISH (BAC-FISH or YAC-FISH); (5) Tyramide signal amplification-FISH (TSA-FISH); (6) Polypeptide nucleic acid-FISH (PNA-FISH) and (7) padlock-FISH.

荧光原位杂交技术的发展与应用
王玲 宁顺斌 宋运淳 吕应堂*

（武汉大学植物发育生物学教育部重点实验室，武汉430072）
关键词： 荧光原位杂交；多色荧光原位杂交；DNA 纤维；分辨率；灵敏度；特异性；物理作图；RNA表达图谱

Key words: FISH, multicolor-FISH, DNA fiber, resolution, sensitivity, specificity, physical mapping, RNA expression pattern