Abstract: Chromosomal fluorescence banding in Haynaldia villosa (2n=14),Zea mays (2n=20) and Secale cereale (2n=14) have been studied. Stained with Hoe-chst 33258, all three species show chromosomal banding clearly and brightly, but withquinacrine. 2HCl only the staining of H. villosa chromosomes are satisfactory. While thequinacrine fluorescence banding of maize and Secale is not clearer and quench morequickly. The positions of the Ho-banding are basically identical with the Q-banding. A comparison has been made between the fluorescence banding and the C-banding in great detail. The results show that the two barding patterns are about the same, but there are also some differences ,between them. We have also discussed the reason of the similarities and the differences between Ho-banding, Q-banding and C-banding in chromosomes of same species.

Key words: Chromosome, Eluorescence banding, Haynaldia villosa, Zea mays, Secale cereale