J Integr Plant Biol. ›› 1994, Vol. 36 ›› Issue (7): -.

• Research Articles •    

Chromosomal Ribonucleoproteins at Mitotic Metaphase in Allium sativum

Wang Xue, Guo Shi-yi, Song Lin-sheng, Fan Ting-yu, Wang Xiu-ling and Zhang Zi-li   

Abstract: Chromosomal ultrastructure and ribonucleoproteins (RNP) at mitotic metaphase in garlic (Allium sativum L. ) were studied. Areas of low electron in density were observed in the centre of some chromosomes with double staining with Ag staining technic. These areas of low electronic density were further observed as perferated holes. With Bernhard staining technique RNP could be detected in the holes but not evenly distributed. RNA identification was further ascertained by NaOH-treatment. The amount of RNP visualized in Bernhard staining technique gradually faded as the duration of EDTA treatment was in creased.

Key words: Chromosome, Ribonucleoprotein, Allium sativum

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