OsWR2 recruits HDA704 to regulate the deacetylation of H4K8ac in the promoter of OsABI5 in response to drought stress

doi:10.1111/jipb.13481

J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (7): 1651-1669.DOI: 10.1111/jipb.13481

• Abiotic Stress Responses • Previous Articles Next Articles

OsWR2 recruits HDA704 to regulate the deacetylation of H4K8ac in the promoter of OsABI5 in response to drought stress

Yalu Guo^1,2†, Yiqing Tan^3†, Minghao Qu^4†, Kai Hong³, Longjun Zeng⁵, Lei Wang⁶, Chuxiong Zhuang², Qian Qian⁷, Jiang Hu^7* and Guosheng Xiong^3*

1. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China;
2. Guangdong Laboratory for Lingnan Modern Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro‐bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;
3. Plant Phenomics Research Center, Academy of Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing 210095, China;
4. College of Animal Science and Technology, Southwest University, Chongqing 402460, China;
5. Yichun Academy of Sciences, Yinchun 336000, China;
6. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, China;
7. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310000, China
^†These authors contributed equally to this work.
^*Correspondences: Guosheng Xiong (gsxiong@njau.edu.cn, Dr. Xiong is fully responsible for the distributions of the materials associated with this article); Jiang Hu (Hujiang588@163.com)

Received:2022-12-04 Accepted:2023-03-13 Online:2023-03-15 Published:2023-07-01

Abstract

Abstract: Drought stress is a major environmental factor that limits the growth, development, and yield of rice (Oryza sativa L.). Histone deacetylases (HDACs) are involved in the regulation of drought stress responses. HDA704 is an RPD3/HDA1 class HDAC that mediates the deacetylation of H4K8 (lysine 8 of histone H4) for drought tolerance in rice. In this study, we show that plants overexpressing HDA704 (HDA704-OE) are resistant to drought stress and sensitive to abscisic acid (ABA), whereas HDA704 knockout mutant (hda704) plants displayed decreased drought tolerance and ABA sensitivity. Transcriptome analysis revealed that HDA704 regulates the expression of ABA-related genes in response to drought stress. Moreover, HDA704 was recruited by a drought-resistant transcription factor, WAX SYNTHESIS REGULATORY 2 (OsWR2), and co-regulated the expression of the ABA biosynthesis genes NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3), NCED4, and NCED5 under drought stress. HDA704 also repressed the expression of ABA-INSENSITIVE 5 (OsABI5) and DWARF AND SMALL SEED 1 (OsDSS1) by regulating H4K8ac levels in the promoter regions in response to polyethylene glycol 6000 treatment. In agreement, the loss of OsABI5 function increased resistance to dehydration stress in rice. Our results demonstrate that HDA704 is a positive regulator of the drought stress response and offers avenues for improving drought resistance in rice.

Key words: drought stress, HDA704, histone acetylation, OsABI5, OsWR2, rice

Yalu Guo, Yiqing Tan, Minghao Qu, Kai Hong, Longjun Zeng, Lei Wang, Chuxiong Zhuang, Qian Qian, Jiang Hu and Guosheng Xiong. OsWR2 recruits HDA704 to regulate the deacetylation of H4K8ac in the promoter of OsABI5 in response to drought stress[J]. J Integr Plant Biol., 2023, 65(7): 1651-1669.

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OsWR2 recruits HDA704 to regulate the deacetylation of H4K8ac in the promoter of OsABI5 in response to drought stress

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