J Integr Plant Biol. ›› 2007, Vol. 49 ›› Issue (7): -.DOI: 10.1111/j.1672-9072.2007.00486.x

• Development & Photosynthesis •    

Mutation of Residue Arginine18 of Cytochrome b55α-Subunit and its Effects on Photosystem II Activities in Chlamydomonas reinhardtii

Jing-Jing Ma, Liang-Bi Li, Yu-Xiang Jing and Ting-Yun Kuang   

Abstract: It has been known that arginine is used as the basic amino acid in the α-subunit of cytochrome b559 (Cyt b559) except histidine. However, previous studies have focused on the function of histidine in the activities of photosystem (PS) II and there are no reports regarding the structural and/or functional roles of arginine in PSII complexes. In the present study, two arginine18 (R18) mutants of Chlamydomonas reinhardtii were constructed using site-directed mutagenesis, in which R18 was replaced by glutamic acid (E) and glycine (G). The results show that the oxygen evolution of the PSII complex in the R18G and R18E mutants was approximately 60% of wild-type (WT) levels and that, after irradiation at high light intensity, oxygen evolution for the PSII of mutants was reduced to zero compared with 40% in WT cells. The efficiency of light capture by PSII (Fv/Fm) of R18G and R18E mutants was approximately 42%–46% that of WT cells. Furthermore, levels of the α-subunit of Cyt b559 and PsbO proteins were reduced in thylakoid membranes compared with WT. Overall, these data suggest that R18 plays a significant role in helping Cyt b559 maintain the structure of the PSII complex and its activity, although it is not directly bound to the heme group.

Key words: Chlamydomonas reinhardtii, chloroplast transformation, cytochrome b559, mutation, photosystem II

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