J Integr Plant Biol. ›› 2005, Vol. 47 ›› Issue (3): -.DOI: 10.1111/j.1744-7909.2005.00027.x

• Research Articles •    

Antiporter Gene from Hordum brevisubulatum (Trin.) Link and Its Overexpression in Transgenic Tobaccos

Shi-You LÜ, Yu-Xiang JING, Shi-Hua SHEN, Hua-Yan ZHAO, Lan-Qing MA, Xiang-Juan ZHOU, Qing REN,Yan-Fang LI   

Abstract: A vacuolar Na+/H+ antiporter cDNA gene was successfully isolated from Hordeum brevisubulatum (Trin.) Link using the rapid amplification of cDNA ends (RACE) method. The gene was named HbNHX1 and was found to consist of 1 916 bp encoding a predicted polypeptide of 540 amino acids with a conserved amiloride-binding domain. Phylogenetic tree analysis of the Na+/H+ antiporters showed that the HbNHX1 gene shares 55.3%–74.8% similarity with the vacuolar-type Na+/H+ antiporters. Transgenic tobaccos that contain the HbNHX1 gene, integrated by forward insertion into the tobacco genome, were obtained via Agrobacterium tumerfaciens and characterized for the determination of the concentration of Na+ and K+ ions, as well as proline, in the presence of 300 mmol/L NaCl. The T1 transgenic plants showed more tolerance to salt and drought than did wild-type plants. Our data suggest that overexpression of the HbNHX1 gene could improve the tolerance of transgenic tobaccos to salt and drought through the function of the vacuolar Na+/H+ antiporter.

Key words: HbNHX1 gene, Hordeum brevisubulatum (Trin.) Link, salt stress, tobacco, vacuolar Na+/H+ antiporter.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q