J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (3): 292-296.

• Research Articles • Previous Articles     Next Articles

Relationship Between H+-ATPase Activity and Fluidity of Tonoplast in Barley Roots Under NaCl Stress

ZHANG Wen Hua, CHEN Qin, LIU You Liang   

Abstract:

H+ ATPase activity of tonoplast in roots of Hordeum vulgare L. cv.“Tanyin 2" (salt tolerant cultivar) increased when the roots were exposed to 50-200 mmol/L NaCl for 2 d, and decreased when NaCl concentration was increased to 600 mmol/L. In “Kepin 7" (salt sensitive cultivar), tonoplast H+ ATPase activity in roots also increased at lower levels of NaCl (50-100 mmol/L), but decreased at higher levels of NaCl (200-600 mmol/L). Tonoplast fluidity in roots of“Tanyin 2" decreased at 50-200 mmol/L NaCl, and increased significantly at 600 mmol/L NaCl. Under salt stress, the change of tonoplast fluidity was identical with that of the ratio of unsaturated fatty acids to saturated fatty acids in tonoplast lipid of barley roots. It is proposed that the increase of tonoplast fluidity due to increased degree of unsaturation of fatty acids is one of the reasons leading to the decrease of H+ ATPase activity under higher level of NaCl stress.

NaCl胁迫下大麦根系液泡膜H+-ATPase活性与膜流动性的关系

章文华1  陈沁2  刘友良1

(1.南京农业大学农学院,南京,2100952.上海大学生命科学学院,上海,200436)

摘要:用50200 mmol/L NaCl处理2 d,大麦(Hordeum vulgare L.)品种"滩引2"(耐盐性强)根的液泡膜H+-ATPase活性增强,600 mmol/L NaCl处理下酶活性下降;"科品7"(耐盐性弱)50100 mmol/L NaCl处理2 d后根的液泡膜H+-ATPase活性增强,200600 mmol/L NaCl处理下酶活性随盐浓度增加而降低.50200 mmol/L NaCl处理下"滩引2"根的液泡膜流动性下降,600 mmol/L NaCl处理下膜流动性明显增大;盐胁迫下液泡膜膜脂脂肪酸不饱和度下降时,膜流动性下降,反之则膜流动性上升.由此推断高盐胁迫下液泡膜膜脂脂肪酸不饱和度上升而引起膜流动性上升可能是引起H+-ATPase活性下降的原因之一.

关键词 盐胁迫 H+-ATPase 膜流动性 脂肪酸组分 大麦

 

Key words: salt stress, H+ ATPase, membrane fluidity, fatty acid composition, barley

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