Abstract:

Nine rice Oryza sativa L.） mutant lines lacking the seed storage glutelin α-2 subunit were obtained from the progenies of fertilized egg cells treated with N-methy-N-nitrosourea (MNU). The mutants could be classified into three types: the α-1 subunit increased type (α-1H/α-2L), decreased the β-2 subunit decreased type (β-2L/α-2L) and the α-3 subunit increased type (α-3H/α-2L) according to their SDS-PAGE profiles. Two-dimensional electrophoresis analysis revealed that all of the mutants lacked a polypeptide of pI 6.71/α-2, while new polypeptides of pI 6.50/α-1 and pI 6.90/α-3 formed in α-1H/α-2L and α-3H/α-2L mutants respectively. Although the β-2L/α-2L mutants did not form new polypeptide, their pI 8.74/β-2 polypeptide was also decreased, suggesting that the two polypeptides decreased in β-2L/α-2L mutants might derive from the same glutelin precursor. These mutant lines are very useful in studying genetic characterisation,the mechanism of genetic regulation on biosynthesis, gene function and proteomics of rice seed storage glutelin.

水稻种子贮藏谷蛋白a-2亚基减少突变体
曲乐庆¹ 魏晓丽¹ 佐藤光² 小川雅广³  熊丸敏博²

（1. 中国科学院遗传研究所，北京100101；2.  日本国立九州大学农学部，福冈812-8581；3. 日本国山口县立大学生命科学部，山口753-8502

摘要：通过筛选水稻（Oryza sativa L.)受精卵的甲基亚硝基脲（MNU）处理后代，获得9个谷蛋白α-2亚基减少（α－2L）突变体。这些突变体依据其SDS－PAGE图谱又可分成3种类型：α－1显著增加型（α－1H／α－2L）、β－2减少型（β－2L／α－2L）和α－3显著增加型（α3－H／α－2L）。双向电泳分析揭示了产生突变体α-2L的原因在于缺少了一条pI6.71/α－2的多肽；α－1H和α－3H的原因在于分别敢一条新的多肽pI6.50/α-1和pI6.90/α-3;而产生β－2的原因在于缺少一条pI18.74/β－2的多肽。pI6.71/α-2和pI8.74/β-2多肽同时缺失于同一突变体暗示二者可能来源于同一前本，为同一基因的产物。这些突变体为水稻谷蛋白遗传规律、生物合成遗传调控机制、基因功能以及蛋白组学研究不可多得的素材。

关键词： 谷蛋白；突变体；双向电泳；水稻种子

Key words: glutelin, mutant, two-dimensional electrophoresis, rice seed