J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (2): 151-161.DOI: 10.1111/jipb.12230

Special Issue: Rice Genomics and Agriculture

• Molecular Physiology • Previous Articles     Next Articles

Identification and characterization of Mini1, a gene regulating rice shoot development

Yunxia Fang1,2†, Jiang Hu1†, Jie Xu1,3†, Haiping Yu1, Zhenyuan Shi1, Guosheng Xiong4, Li Zhu1, Dali Zeng1, Guangheng Zhang1, Zhenyu Gao1, Guojun Dong1, Meixian Yan1, Longbiao Guo1, Yonghong Wang4 and Qian Qian1*   

  1. 1State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
    2College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China
    3Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, China
    4State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, Beijing, China
  • Received:2014-04-11 Accepted:2014-06-18 Published:2014-06-19
  • About author:These authors contributed equally to this work.
    *Correspondence: E-mail: qianqian188@hotmail.com


The aerial parts of higher plants are generated from the shoot apical meristem (SAM). In this study, we isolated a small rice (Oryza sativa L.) mutant that showed premature termination of shoot development and was named mini rice 1 (mini1). The mutant was first isolated from a japonica cultivar Zhonghua11 (ZH11) subjected to ethyl methanesulfonate (EMS) treatment. With bulked segregant analysis (BSA) and map-based cloning method, Mini1 gene was finally fine-mapped to an interval of 48.6 kb on chromosome 9. Sequence analyses revealed a single base substitution from G to A was found in the region, which resulted in an amino acid change from Gly to Asp. The candidate gene Os09g0363900 was predicted to encode a putative adhesion of calyx edges protein ACE (putative HOTHEAD precursor) and genetic complementation experiment confirmed the identity of Mini1. Os09g0363900 contains glucose-methanol-choline (GMC) oxidoreductase and NAD(P)-binding Rossmann-like domain, and exhibits high similarity to Arabidopsis HOTHEAD (HTH). Expression analysis indicated Mini1 was highly expressed in young shoots but lowly in roots and the expression level of most genes involved in auxin biosynthesis and signal transduction were reduced in mutant. We conclude that Mini1 plays an important role in maintaining SAM activity and promoting shoot development in rice.


Fang Y, Hu J, Xu J, Yu H, Shi Z, Xiong G, Zhu L, Zeng D, Zhang G, Gao Z, Dong G, Yan M, Guo L, Wang Y, Qian Q (2015) Identification and characterization of Mini1, a gene regulating rice shoot development. J Integr Plant Biol 57: 151–161. doi: 10.1111/jipb.12230

Key words: HOTHEAD, mini, rice, shoot development

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