Please wait a minute...
J Integr Plant Biol, 2016, 58 (12): 971-982, Research Article
RLS3, a protein with AAA+ domain localized in chloroplast, sustains leaf longevity in rice
Yanhui Lin, Lubin Tan, Lei Zhao, Xianyou Sun and Chuanqing Sun*
State Key Laboratory of Plant Physiology and Biochemistry, National Center for Evaluation of Agricultural Wild Plants (Rice), MOE Key Laboratory of Crop Heterosis and Utilization, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China
*Correspondence: E-mail: Chuanqing Sun (suncq@cau.edu.cn)
doi: 10.1111/jipb.12487
Abstract

Leaf senescence plays an important role in crop developmental processes that dramatically affect crop yield and grain quality. The genetic regulation of leaf senescence is complex, involving many metabolic and signaling pathways. Here, we identified a rapid leaf senescence 3 (rls3) mutant that displayed accelerated leaf senescence, shorter plant height and panicle length, and lower seed set rate than the wild type. Map-based cloning revealed that RLS3 encodes a protein with AAA+ domain, localizing it to chloroplasts. Sequence analysis found that the rls3 gene had a single-nucleotide substitution (G[RIGHTWARDS ARROW]A) at the splice site of the 10th intron/11th exon, resulting in the cleavage of the first nucleotide in 11th exon and premature termination of RLS3 protein translation. Using transmission electron microscope, the chloroplasts of the rls3 mutant were observed to degrade much faster than those of the wild type. The investigation of the leaf senescence process under dark incubation conditions further revealed that the rls3 mutant displayed rapid leaf senescence. Thus, the RLS3 gene plays key roles in sustaining the normal growth of rice, while loss of function in RLS3 leads to rapid leaf senescence. The identification of RLS3 will be helpful to elucidate the mechanisms involved in leaf senescence in rice.

--------------------------------------------------------------------------------------------------------------------
Received: 22 April 2016      Accepted:    Online on:
This Article
   Full Text  
   Full Text (PDF) 
   Supporting information
Services
   E-mail Link to this Article
   Export Citation for this Article
Citing Articles
   Cited By
Google Scholar
   Articles by Yanhui Lin
   Articles by Lubin Tan
   Articles by Lei Zhao
   Articles by Xianyou Sun
   Articles by Chuanqing Sun
PubMed
   Articles by Yanhui Lin
   Articles by Lubin Tan
   Articles by Lei Zhao
   Articles by Xianyou Sun
   Articles by Chuanqing Sun
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22