J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (4): 379-382.

• Research Articles • Previous Articles     Next Articles

Morphological and Anatomical Analyses of a Floral Organ Mutant in Rice

WANG Wen Ming, XING Shao Chen , ZHENG Xian Wu, ZHAO Xian Feng, ZHU Li Huang   


A twinovary mutant derived from the doubled haploid (DH) progeny of a cross,02428/Gui 630, was presumably related to the transposition of some transposable elements. Up to date, all reported the active transposable elements in rice (Oryza sativaL.) are copia like retrotransposons. In the present study, the reverse transcriptase domains of copia like retrotransposons were amplified from the total DNA isolted from the mutant plants with the degenerated oligonucleotide primers for the domain. Three cloned insert DNAs, R331, R334 and R338, representing putative different copialike retrotransposons were screened out. Two of them displayed high polymorphism between “Zhaiyeqing 8” and “Jingxi 17”. Nine of the polymorphic bands were mapped on seven rice chromosomes. Sequencing analysis revealed that stop codons frequently occur in the sequence of R338, while both R331 and R334 contain a continuous coding region for 81 putative amino acid residues. No significant variation in hybridization patterns was found between indica and japonica rice or among 26 varieties of indica rice when R33 1 was used as a probe. Nevertheless, when R33 4 was used as a probe, high polymorphisms were detected both between indica and japonica rice and among 26 indica varieties, implying that this element might still be active in rice genomes.

王文明1 朱立煌1*  谢戎2  谢灿1  金良2

(1. 中国科学院植物生物技术开放实验室,北京100101;2.  四川省农业科学院水稻高粱研究所,四川泸州646000)

摘要:从水稻(Oryza sativa L.)育种后代材料中获得一个受单隐性基因控制的颖花器官突变体,该突变体的内外释变,每个颖花含1~3个发育完整的雌蕊,其他雌蕊发育不完全。有的雌蕊化的雄蕊下部为花丝,其上着生不规则在组织和0~3个羽毛状柱头。横切面和纵切面观察均可见每个颖花含3个子房。人工辅助授粉后,每个颖花可产生1~2粒种子根据这些结果推断,该突变体为类似B功能缺失的突变体。

关键词: 水稻;花器官;B功能缺失;突变体

Key words: rice, copia like retrotransposon, transposable element, reverse transcriptase, transposition

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