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J Integr Plant Biol, 2000, 42 (9): 920-926, Research Article
Effects of Salinity Stress on the Levels of Covalently and Noncovalently Conjugated Polyamines in Plasma Membrane and Tonoplast Isolated from Barley Seedlings
Zhao Fu-Geng

When the 7-d old barley ( Hordeum vulgare L. ) seedlings were treated with different concentrations of NaC1 for 3 d, the levels of the noncovalemly conjugated polyamines (PAs) in the plasma membrane and tonoplast vesicles and the covalently conjugated PAs in the membrane proteins were promoted by NaC1 of low concentrations and suppressed by NaC1 of high concentrations. Among the noncovalenfly conjugated PAs in the vesicles, spennidine (Spd) level was the most abundant, while putrescine (Put) content was predominant among the covalenfly conjugated PAs, accounted for 40%– 70%, 35% – 60%, respectively. In addition, the TLC (thin-layer chromatography) profiles of the benzoylated PAs presented an unknown polyamine with Rf = 0.92 (X0.92), which conjugated covalently and noncovalently in root tonoplast and its content changed as well as Spd with NaC1 treatment. The total PA contents in the roots were higher than that in the leaves, and the types and contents of covalenfly and noncovalently conjugated PAs in the tonoplast were higher than those in the plasma membrane. The results showed that the above two PAs associated with the membrane might be essential in salt adaption of cells and the maintenance of membrane function.

赵福庚 孙诚 刘友良* 刘兆普

摘要:用不同浓度NaCl处理 7d龄大麦 (Hordeumvulgare L .)幼苗 3d ,以非共价键和共价键形式分别与质膜和液泡膜微囊及膜蛋白结合的多胺含量受低浓度盐的促进而被高浓度盐所抑制。以非共价键形式与膜微囊结合的各种多胺中亚精胺 (Spd)含量最高 ,占膜上多胺总量的 40 %~ 70 %。与膜蛋白共价结合的各种多胺中腐胺 (Put)含量占主导地位 ,占膜蛋白上多胺总量的 35 %~ 6 0 %。在根系液泡膜上发现一种含量丰富的未知多胺X0 .92 ,该多胺以非共价键和共价键两种形式与膜结合 ,随NaCl处理浓度的变化趋势与Spd相同。根系膜微囊上多胺总含量大于叶片。盐胁迫下液泡膜微囊上两种结合形式多胺种类及含量的变化幅度均大于质膜。表明上述两种膜结合多胺可能在细胞的盐适应和膜功能维持中起重要作用。

关键词: 质膜;液泡膜;多胺;盐胁迫;大麦

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