Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H2 evolution

doi:10.1111/jipb.12502

J Integr Plant Biol. ›› 2016, Vol. 58 ›› Issue (12): 943-946.DOI: 10.1111/jipb.12502 cstr: 32098.14.jipb.12502

• Letter to the Editor • Next Articles

Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H₂ evolution

Mei Chen^1†, Jin Zhang^1,2†, Lei Zhao¹, Jiale Xing^1,2, Lianwei Peng¹, Tingyun Kuang¹, Jean-David Rochaix³ and Fang Huang^1*

¹Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China
²University of Chinese Academy of Sciences, Beijing 100049, China
³Departments of Molecular Biology and Plant Biology, University of Geneva, 1211 Geneva, Switzerland

Received:2016-09-23 Accepted:2016-10-14 Published:2016-10-20
About author:^†These authors contributed equally to this work.
^*Correspondence: E-mail: fhuang@ibcas.ac.cn

Abstract

Abstract:

We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5 (PGR5) capable of producing hydrogen (H₂) for 25 days with more than 30-fold yield increase compared to wild type. Thus, hpm91 displays a higher capacity of H₂ production than a previously characterized pgr5 mutant. Physiological and biochemical characterization of hpm91 reveal that the prolonged H₂ production is due to enhanced stability of PSII, which correlates with increased reactive oxygen species (ROS) scavenging capacity during sulfur deprivation. This anti-ROS response appears to protect the photosynthetic electron transport chain from photo-oxidative damage and thereby ensures electron supply to the hydrogenase.

Key words: Chlamydomonas reinhardtii, hpm91, hydrogen photoproduction, oxidative stress, photosystem II, sulfur deprivation

Mei Chen, Jin Zhang, Lei Zhao, Jiale Xing, Lianwei Peng, Tingyun Kuang, Jean-David Rochaix, and Fang Huang. Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H₂ evolution[J]. J Integr Plant Biol., 2016, 58(12): 943-946.

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Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H₂ evolution

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