Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H2 evolution

doi:10.1111/jipb.12502

J Integr Plant Biol. ›› 2016, Vol. 58 ›› Issue (12): 943-946.DOI: 10.1111/jipb.12502

• Letter to the Editor • Next Articles

Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H₂ evolution

Mei Chen^1†, Jin Zhang^1,2†, Lei Zhao¹, Jiale Xing^1,2, Lianwei Peng¹, Tingyun Kuang¹, Jean-David Rochaix³ and Fang Huang^1*

¹Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China
²University of Chinese Academy of Sciences, Beijing 100049, China
³Departments of Molecular Biology and Plant Biology, University of Geneva, 1211 Geneva, Switzerland

Received:2016-09-23 Accepted:2016-10-14 Published:2016-10-20
About author:^†These authors contributed equally to this work.
^*Correspondence: E-mail: fhuang@ibcas.ac.cn

Abstract

Abstract:

We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5 (PGR5) capable of producing hydrogen (H₂) for 25 days with more than 30-fold yield increase compared to wild type. Thus, hpm91 displays a higher capacity of H₂ production than a previously characterized pgr5 mutant. Physiological and biochemical characterization of hpm91 reveal that the prolonged H₂ production is due to enhanced stability of PSII, which correlates with increased reactive oxygen species (ROS) scavenging capacity during sulfur deprivation. This anti-ROS response appears to protect the photosynthetic electron transport chain from photo-oxidative damage and thereby ensures electron supply to the hydrogenase.

Key words: Chlamydomonas reinhardtii, hpm91, hydrogen photoproduction, oxidative stress, photosystem II, sulfur deprivation

Mei Chen, Jin Zhang, Lei Zhao, Jiale Xing, Lianwei Peng, Tingyun Kuang, Jean-David Rochaix, and Fang Huang. Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H₂ evolution[J]. J Integr Plant Biol., 2016, 58(12): 943-946.

[1]	Sha Yang, Fang Wang, Feng Guo, Jing-Jing Meng, Xin-Guo Li, and Shu-Bo Wan. Calcium contributes to photoprotection and repair of photosystem II in peanut leaves during heat and high irradiance [J]. J Integr Plant Biol., 2015, 57(5): 486-495.
[2]	Xiao-Yan Xu, Zhong-Jie Ding, Lei Chen, Jin-Ying Yan, Gui-Xin Li, and Shao-Jian Zheng. An eukaryotic translation initiation factor, AteIF5A-2, affects cadmium accumulation and sensitivity in Arabidopsis [J]. J Integr Plant Biol., 2015, 57(10): 848-858.
[3]	Yi Gan, Hong Li, Ye Xie, Wenjuan Wu, Maoyin Li, Xuemin Wang, and Jirong Huang. THF1 mutations lead to increased basal and wound-induced levels of oxylipins that stimulate anthocyanin biosynthesis via COI1 signaling in Arabidopsis [J]. J Integr Plant Biol., 2014, 56(9): 916-927.
[4]	Haomeng Yang, Libing Liao, Tingting Bo, Lei Zhao, Xuwu Sun, Xuefeng Lu, Birgitta Norling, and Fang Huang. Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem II under high-light condition [J]. J Integr Plant Biol., 2014, 56(12): 1136-1150.
[5]	Fanying Kong, Yongsheng Deng, Guodong Wang, Jieru Wang, Xiaoqing Liang and Qingwei Meng. LeCDJ1, a chloroplast DnaJ protein, facilitates heat tolerance in transgenic tomatoes [J]. J Integr Plant Biol., 2014, 56(1): 63-74.
[6]	Lei Zhao, Mei Chen, Dongmei Cheng, Haomeng Yang, Yongle Sun, Heyi Zhou, and Fang Huang. Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress [J]. J Integr Plant Biol., 2013, 55(11): 1054-1068.
[7]	Huijuan Zhang, Jing Zhai, Jibo Mo, Dayong Li and Fengming Song. Overexpression of Rice Sphingosine-1-Phoshpate Lyase Gene OsSPL1 in Transgenic Tobacco Reduces Salt and Oxidative Stress Tolerance [J]. J Integr Plant Biol., 2012, 54(9): 652-662.
[8]	Marta Juvany, Maren Müller and Sergi Munné-Bosch. Leaves of Field-Grown Mastic Trees Suffer Oxidative Stress at the Two Extremes of their Lifespan [J]. J Integr Plant Biol., 2012, 54(8): 584-594.
[9]	Richard P. Jacoby, Lei Li, Shaobai Huang, Chun Pong Lee, A. Harvey Millar, and Nicolas L. Taylor. Mitochondrial Composition, Function and Stress Response in Plants [J]. J Integr Plant Biol., 2012, 54(11): 887-906.
[10]	Xiao-Yue Chen, Xiao Ding, Sheng Xu, Ren Wang, Wei Xuan, Ze-Yu Cao, Jian Chen, Hong-Hong Wu, Mao-Bing Ye, and Wen-Biao Shen. Endogenous Hydrogen Peroxide Plays a Positive Role in the Upregulation of Heme Oxygenase and Acclimation to Oxidative Stress in Wheat Seedling Leaves [J]. J Integr Plant Biol., 2009, 51(10): 951-960.
[11]	Jing-Jing Ma, Liang-Bi Li, Yu-Xiang Jing and Ting-Yun Kuang. Mutation of Residue Arginine18 of Cytochrome b55α-Subunit and its Effects on Photosystem II Activities in Chlamydomonas reinhardtii [J]. J Integr Plant Biol., 2007, 49(7): -.
[12]	Zhi-Sheng Sa, Li-Qin Huang, Guo-Lin Wu, Jin-Peng Ding, Xiao-Yue Chen,Tian Yu, Ci Shi and Wen-Biao Shen. Carbon Monoxide: A Novel Antioxidant Against Oxidative Stress in Wheat Seedling Leaves [J]. J Integr Plant Biol., 2007, 49(5): -.
[13]	Yunlai Tang, Mei Chen, Yinong Xu and Tingyun Kuang. Changes in Thermostability of Photosystem II and Leaf Lipid Composition of Rice Mutant with Deficiency of Light-harvesting Chlorophyll a/b Protein Complexes [J]. J Integr Plant Biol., 2007, 49(4): -.
[14]	Rajesh Kumar Tewari, Praveen Kumar and Parma Nand Sharma. Oxidative Stress and Antioxidant Responses in Young Leaves of Mulberry Plants Grown Under Nitrogen, Phosphorus or Potassium Deficiency [J]. J Integr Plant Biol., 2007, 49(3): -.
[15]	Yi Han, Wei Xuan, Tian Yu, Wen-Bing Fang, Tian-Ling Lou, Yin Gao, Xiao-Yue Chen, Xiao Xiao and Wen-Biao Shen. Exogenous Hematin Alleviates Mercury-induced Oxidative Damage in the Roots of Medicago sativa [J]. J Integr Plant Biol., 2007, 49(12): 1703-1713.

Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H₂ evolution

HTML

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments