Abstract:

A double haploid (DH) population of rice ( Oryza sativa L.) derived from anther culture of ZYQ8/JX17, a typical indica and japonica hybrid, was used for genetic analysis of rice peduncle vascular system and panicle traits. The number of large vascular bundles (LVB), the number of small vascular bundles (SVB) in the peduncle, and the panicle traits including the number of primary rachis branches (PRB), the number of spikelets per panicle (SNP), peduncle top diameter (PTD), and panicle length (PL) were investigated in the parents and DH lines. The quantitative trait loci (QTLs) for each trait were analyzed based on the constructed molecular linkage map of this population. Three QTLs for LVB (qLVB 1, qLVB 6 and qLVB 7) were detected on chromosomes 1, 6, and 7, respectively. Two putative QTLs for SVB (qSVB 4 and qSVB 6) were mapped on chromosomes 4 and 6 respectively. Four QTLs (qPRB 4a, qPRB 4b, qPRB 6 and qPRB 7) on chromosomes 4, 6, and 7, respectively, were detected for PRB. Three QTLs (qSPN 4a, qSPN 4b and qSPN 6) were identified on chromosomes 4 and 6, respectively, which could significantly affect SPN. Five QTLs for PTD (qPTD 2, qPTD 5, qPTD 6, qPTD 8 and qPTD 12) were identified on chromosomes 2, 5, 6, 8, and 12, respectively. Three QTLs for PL (qPL 4, qPL 6 and qPL 8) were detected on chromosomes 4, 6, and 8, respectively. Clustering of QTLs, such as qLVB 6, qSVB 6, qSNP 6, qPTD 6, and qPL 6 detected in the interval G122 G1314b on chromosome 6, was found. These results suggest that some QTLs for peduncle vascular bundle system are possibly responsible for the panicle traits.

水稻穗颈维管束及穗部性状的QTL分析

滕胜^1,3  钱前¹  曾大力¹  国广泰史⁴  黄大年^1,3  朱立煌^2*

(1.中国水稻研究所农业部水稻生物学重点实验室,杭州,310006 ； 2. 中国科学院遗传研究所，北京 100101; 3.浙江大学生命科学学院,杭州,310029 ；4.日本国际农林水产研究中心,日本筑波)

摘要：以籼稻(Oryza sativa L. ssp. indica ZYQ8)和粳稻(O. sativa ssp. japonica JX17)的杂交F1代花培加倍的DH群体为材料考察了该群体的穗颈节大小维管束数、一次枝梗数、每穗颖花数、穗颈节顶部直径和穗长,并用该群体构建的分子图谱进行数量性状座位(QTL)分析.检测到控制大维管束的3个QTL (qLVB-1、qLVB-6和qLVB-7)分别位于第1、第6和第7染色体;控制小维管束的2个QTL (qSVB-4和qSVB-6)分别位于第4和第6染色体;控制一次枝梗的4个QTL (qPRB-4a、qPRB-4b、qPRB-6和qPRB-7)分别位于第4 (2个)、第6和第7染色体;每穗颖花数的3个QTL (qSPN-4a、qSPN-4b和 qSPN-6)分别位于第4 (2个)和第6染色体上;穗颈节顶部直径的5个QTL (qPTD-2、qPTD-5、qPTD-6、qPTD-8和qPTD-12)分别位于第2、第5、第6、第8和第12染色体;穗长的3个QTL (qPL-4、qPL-6和qPL-8)分别位于第4、第6、第8染色体上.其中qLVB-6、qSVB-6、qSPN-6、qPTD-6和qPL-6均位于第6染色体的G122-G1314b之间;qPL-8和qPTD-8位于第8染色体的GA408-BP127a之间;qPRB-4a和qSPN-4a位于第4染色体的G177-CT206之间;qPL-4和qSPN-4b位于第4染色体CT404-CT500之间;qSVB-4所在的区间与qPL-4、qSPN-4b和qPRB-4b所在的区间相邻.

关键词 水稻 穗颈维管束 穗部性状 QTL定位

*通讯作者 Email: lhzhu@ss10.igtp.ac.cn

Key words: rice, peduncle vascular bundle, panicle traits, QTL analysis